![]() The statistical significance of the difference between the control and treated groups was evaluated using a one-way ANOVA. Trypan blue as vital staining was used for viability. Neutrophil-like cells were incubated with LPS (200 ng/ml) for 45 min, followed by incubation for 25 min with 100 ng/ml Hoechst 33342. Flow cytometry was used to evaluate CD11b in the differentiated cells, and the NBT assay was used to evaluate the functionality of the differentiated HL-60 cells. Methods: In this study, the HL60 cell line was used for culture and DMSO for induction and differentiation. ![]() This study focused on whether LPS induced NETs release in vitro in the HL60 cell line. NETs is a new strategy for pathogen response. Recently, a novel strategy called the formation neutrophil extracellular traps (NETs) was described. Background: Neutrophils are the most abundant white blood cells in humans.
0 Comments
Leave a Reply. |